Tetrapleura tetraptera Extract Inhibited Luteinizing Hormone and Estrogen Secretion in Clomiphene Citrate Treated Female Wistar Albino Rats

Tetrapleura tetraptera stem bark has been reported to cause inhibition of luteinizing hormone release in cultured rat pituitary cells. Hence, we investigated the effects of Tetraptera tetrapleura pod extract on follicle stimulating hormone, luteinizing hormone, cortisol, progesterone and estrogen. Thirty non-pregnant female wistar albino rats were divided into group A - D. Group A rats were used as Control. Group B rats were administered 1 mg/kg/day of clomiphene citrate orally. Group C rats were administered 200 mg/kg of extract only, whereas group D rats were administered 1 mg/kg /day of Clomiphene citrate plus 200 mg/kg of extract. At the end of 14 days experiment, group A, B and D were found in proestrus phase and group C in diestrus phase. FSH and cortisol levels remained unchanged. Group C and D rats produced significant reduction (P < 0.05) in LH and estrogen levels in prolonged proestrus and normal diestrus respectively. Progesterone level was significantly high (P < 0.05) in the group C rats. The reduced LH level could be due to the anti-estrogenic effect of extract during proestrus when LH secretion is expected to surge. But co-administration resulted in high progesterone secretion, suggesting extract may have influenced progesterone secretion in group D rats simultaneously administered with clomiphene citrate and extract. The above findings indicated that Tetrapleura tetraptera pod extracts inhibited luteinizing hormone and estrogen even when co-administered with clomiphene citrate.