In vitro Antibacterial Activity of Maclura tinctoria and Azadirachta indica against Streptococcus mutans and Porphyromonas gingivalis

Introduction: Azadirachta indica and Maclura tinctoria are two plants that have been reported with some ability to inhibit the growth of different types of bacteria so the aim of this project was to evaluate the in vitro antibacterial activity of the extract of Maclura tinctoria and Azadirachta indica on Streptococcus mutans and Porphyromonas gingivalis.
Methods: Ethanol extracts were obtained from Azadirachta indica and Maclura tinctoria. Antibacterial activity was evaluated on S. mutans (ATCC25175) and P. gingivalis (ATCC33277) determining the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) using broth dilution tests. An analysis of variance (ANOVA) was performed followed by Dunnett's post-test for multiple comparisons, considering the value P < 0.05.
Results: Azadirachta indica showed a MIC of 500 Ppm (µg/mL) for both bacteria, showing bacteriostatic activity. M. tinctoria at a concentration of 125 Ppm (µg/mL had a bacteriostatic activity and bactericidal at higher concentrations of 250 Ppm (µg/mL and 500 Ppm (µg/mL for S. mutans. Against P. gingivalis the extract presented a MIC and a MCB of 500 Ppm µg/mL).

Discussion: Azadirahcta’s indica and Maclura’s tinctoria ethanol extracts showed inhibitory activity against S. mutans (ATCC25175) and P. gingivalis (ATCC33277) of considerable value. Therefore it is recommended to continue with further studies.